Botanical Gazette Volume 34 epub

Botanical Gazette Volume 34 epub

Botanical Gazette Volume 34.cUniversity of Chicago

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Author: University of Chicago
Page Count: 164 pages
Published Date: 06 Aug 2012
Publisher: Rarebooksclub.com
Publication Country: Miami Fl, United States
Language: English
ISBN: 9781235774027
Download Link: Botanical Gazette Volume 34
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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1902 edition. Excerpt: ... Botanical Gazette OCTOBER, 1902 A MORPHOLOGICAL STUDY OF THUJA. CONTRIBUTIONS FROM THE HULL BOTANICAL LABORATORY. XXXIX. W. J. G. Land. (with Plates Vi-viii) Material for the study of Thuja occidentalis L. was collected in the vicinity of Chicago during the late spring and early summer of 1901. A few collections were made in June 1902, in order to determine whether the time of fertilization varies in two successive seasons. The trees were visited twice each week and material fixed immediately after the removal of the branches. Branches were also kept in the laboratory, some having water pressure applied to the cut end of the stem, and collections were made daily. All the material from which illustrations are made was taken from a single tree, the most vigorous one to be found. Collections were made from many other trees in order to determine whether the periods of the various stages of development are the same in different individuals of the same locality. Little difference could be observed. For stages before the formation of archegonium initials 1 per cent, chromo-acetic acid was most useful, but for later stages it was not so good, as it was not possible to secure rapid penetration of the tissues even when used at a temperature of 80 . A modification of Carnoy's formula was found to give the best results for fertilization and succeeding stages, although many fixing agents were given a thorough trial. None of the fixing agents proved entirely satisfactory. Flemming's triple stain gave good results for pollen tubes and early stages in the development of the archegonia, but by far the most satisfactory stain was Haidenhain's iron alum-haematoxylin. Delafield's haematoxylin combined with orange G was best for embryos. Sections were cut 3 to 5/...

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